abstract：:A combination of two procedures, embedding of specimens and horseradish peroxidase (HRP) histochemistry on mounted serial sections, is proposed for three-dimensional reconstruction of neuronal projections. To clarify the intrinsic organization of the peripheral nervous system, this simple method is more adequate than ...

journal_title：Journal of neuroscience methods

authors： Streinzer W,Krammer EB

更新日期：1986-09-01 00:00:00

abstract：:The procedure described for the continuous release of vasopressin (VP) in the rat lateral ventricle via implantation of a VP-loaded Accurel/collodion mini-device has now been further developed, allowing the introduction, removal and exchange of the Accurel device through a polyethylene guide cannula. In this way, mani...

journal_title：Journal of neuroscience methods

authors： Kruisbrink J,Van der Woude TP,Boer GJ,Mirmiran M

更新日期：1986-08-01 00:00:00

abstract：:A method is presented for the ultrastructural demonstration of succinate semialdehyde dehydrogenase (SSADH) activity in cerebellar Purkinje neurons and liver hepatocytes; SSADH is an enzyme involved in the degradation of gamma-aminobutyric acid (GABA). Incubation media originally used for light microscopy were conside...

journal_title：Journal of neuroscience methods

authors： Bernocchi G,Barni S,Biggiogera M

更新日期：1986-07-01 00:00:00

abstract：:A method is described for the isolation of neurons from defined regions of the mammalian central nervous system, by a combination of mechanical and enzymatic means. The procedure liberates neurons free of cellular debris and glial investments, allowing the formation of giga-ohm seals with patch clamp electrodes. The c...

journal_title：Journal of neuroscience methods

authors： Kay AR,Wong RK

更新日期：1986-05-01 00:00:00

abstract：:An interactive microcomputer system has been developed for the quantitative analysis of Golgi-impregnated neuronal morphology (GINM). The system uses commercially available hardware: a 48K RAM microcomputer (Apple II), a TV monitor (NEC), an optical microscope (Reichert Biovar) with a camera lucida and a specially dev...

journal_title：Journal of neuroscience methods

authors： Freire M

更新日期：1986-04-01 00:00:00

abstract：:A technique has been developed for recording single unit afferent activity from mesenteric nerves in isolated segments of rat distal ileum in vitro. The preparation consists of a 3-cm segment of ileum, containing a single neurovascular bundle, held horizontally in an organ bath. One end of the segment is attached to a...

journal_title：Journal of neuroscience methods

authors： Sharkey KA,Cervero F

更新日期：1986-04-01 00:00:00

abstract：:It is often helpful to display analogue signals such as electromyograms, electroencephalograms, nerve action potentials, etc., in a regular vertical array. A special case of this display is the widely-used dot raster. This paper describes a simple circuit which enables up to 31 analogue signals to be thus displayed on...

journal_title：Journal of neuroscience methods

authors： Miles TS,Woodland MJ

更新日期：1986-03-01 00:00:00

abstract：:A method for studying the process of regeneration in the frog sciatic nerve is presented. The outgrowth of sensory axons was determined by assaying the distribution of axonally transported radioactive proteins by fluorography of whole-mount nerve preparations. After labelling, the nerves were fixed at their in vivo le...

journal_title：Journal of neuroscience methods

authors： Edström A,Sjöberg J,Kanje M

更新日期：1986-03-01 00:00:00

abstract：:Practical aspects on the use of horseradish peroxidase (HRP) and wheat germ agglutinin-horseradish peroxidase conjugate (WGA-HRP) to trace peripheral cutaneous nerve endings have been studied. The parameters studied included application site of the tracer, post-application survival time, tracer concentration and trace...

journal_title：Journal of neuroscience methods

authors： Aldskogius H,Kinnman E,Persson J

更新日期：1986-02-01 00:00:00

abstract：:The feasibility of spindle afferent recording during naturally or electrically induced hindlimb movements is demonstrated in the case of spinalized, decapitated turtles. Since these recordings are obtained from fine filaments of exposed dorsal roots, it is possible for the experimenter to select the afferents of inter...

journal_title：Journal of neuroscience methods

authors： Hasan Z,Sasaki SI

更新日期：1986-02-01 00:00:00

abstract：:Glass plates coated with transparent thin film conductors of indium-tin oxide (ITO), 100 nm thick and 10 microns wide, have been successfully used to record spike potentials from neuronal monolayer cultures. The material is non-toxic to mammalian spinal neurons and is stable under warm culture medium. Laser-deinsulate...

journal_title：Journal of neuroscience methods

authors： Gross GW,Wen WY,Lin JW

更新日期：1985-11-01 00:00:00

abstract：:Electrical potentials recorded extracellularly from the sinus gland of the isopod, Oniscus asellus, were stored on video tape with the aid of a digital-audio (DA) processor and a video cassette recorder (VCR). The DA processor transforms the analog signal to digital pulses of equal amplitude and converts these pulses ...

journal_title：Journal of neuroscience methods

authors： Chiang RG,Knobloch CA,Singleton DM,Steel CG,Davey KG

更新日期：1985-10-01 00:00:00

abstract：:A method for the simultaneous visualization of neurotransmitter receptor binding sites and immunohistochemically characterized cells is described using in vitro sections of rat striatum. The striatum provides a rich neurochemical environment in which to examine muscarinic acetylcholine receptor relations to peptide-re...

journal_title：Journal of neuroscience methods

authors： Ariano MA,Kenny SL

更新日期：1985-10-01 00:00:00

abstract：:The weakly acidic, cation-exchange resin Bio-Rex 70 was used at a low pH for chromatography of enkephalins and other peptides containing enkephalin sequences. Recoveries of various peptide standards and peptides in adrenal chromaffin cell extracts were greater than 85%. Catecholamines and other substances such as dens...

journal_title：Journal of neuroscience methods

authors： Wilson SP

更新日期：1985-10-01 00:00:00

abstract：:Electrophysiological recording provides important evidence for positive identification of many neurons in gastropods. We describe a technique which combines intracellular recording and injection of a persistent, non-fluorescent dye (Fast Green) with subsequent histofluorescence treatment using a modification of the wh...

journal_title：Journal of neuroscience methods

authors： Audesirk TE,Audesirk GJ

更新日期：1985-08-01 00:00:00

abstract：:A novel test for the selective identification of anxiolytic and anxiogenic drug effects in the rat is described, using an elevated + -maze consisting of two open arms and two enclosed arms. The use of this test for detecting such drug effects was validated behaviourally, physiologically, and pharmacologically. Rats ma...

journal_title：Journal of neuroscience methods

authors： Pellow S,Chopin P,File SE,Briley M

更新日期：1985-08-01 00:00:00

abstract：:Since no simple method for standardized positioning of very young rats in a stereotaxic device has been described in the literature, a new method for stereotaxic placement in the neonatal rat was developed, which only requires a simple body mould of the rat pup, and a minor adaptation of a rabbit stereotaxic apparatus...

journal_title：Journal of neuroscience methods

authors： Hoorneman EM

更新日期：1985-07-01 00:00:00

abstract：:A modification of the formalin test appropriate for testing of mice is described. Formalin 1 or 5% was injected into the dorsal surface of a hindpaw, and the time the animal spent licking the paw was recorded. On the basis of the response pattern, two distinct periods of intensive licking activity were identified; an ...

journal_title：Journal of neuroscience methods

authors： Hunskaar S,Fasmer OB,Hole K

更新日期：1985-06-01 00:00:00

abstract：:Computerized control of a bar-press feeding task initiated by a monkey's vocalization was designed to study the motivational roles of brain regions in feeding behavior. In this paradigm, the monkey was required to vocalize in order to initiate the task and then press the bar 30 times to obtain the reward. A microcompu...

journal_title：Journal of neuroscience methods

authors： Yamamoto T,Oomura Y,Nishino H,Aou S,Nakano Y

更新日期：1985-05-01 00:00:00

abstract：:Sources of stereotaxic variability in long-tailed macaques (Macaca fascicularis) were examined by X-ray techniques. Accuracy in the vertical dimension can be improved significantly by measuring from the top of the brain rather than from the Frankfurt plane established by external bony landmarks. Even greater accuracy ...

journal_title：Journal of neuroscience methods

authors： Dubach MF,Tongen VC,Bowden DM

更新日期：1985-04-01 00:00:00

abstract：:A technique has been developed which allows extracellular recording from single neurons in the spinal cord of physiologically intact, awake, drug-free, restrained cats. The technique involves the surgical placement, under general anesthesia, of a stainless steel recording chamber which has a rectangular opening in its...

journal_title：Journal of neuroscience methods

authors： Collins JG

更新日期：1985-02-01 00:00:00

abstract：:We present an efficient algorithm for solving the one-dimensional cable equation in the Laplace (frequency) domain for an arbitrary linear membrane. This method, a reformulation and extension of the geometrical calculus developed by Butz and Cowan (1974), solves for the transfer impedance between any two points in a b...

journal_title：Journal of neuroscience methods

authors： Koch C,Poggio T

更新日期：1985-02-01 00:00:00

abstract：:The rates of deterioration of immunocytochemical staining for 4 peptide and 2 neuronal antigens were studied on human, guinea pig and rat colon and urinary bladder. It was found that if the fixation started within 10 h after surgery or death, little change was seen in the pattern and intensity of the immunostains. Whe...

journal_title：Journal of neuroscience methods

authors： Gu J,Huang WM,Polak JM

更新日期：1985-02-01 00:00:00

abstract：:A program, written in the language Pascal, is described for signal averaging with a microcomputer. The program instructs the computer to acquire data from two channels on-line. For up to 3 channels of extracellular or intracellular recording analysed simultaneously, it was found not to be necessary to employ machine c...

journal_title：Journal of neuroscience methods

authors： Simmons PJ

更新日期：1985-01-01 00:00:00

abstract：:The scanning electron microscope (SEM) is a useful tool in the study of the retina of lower vertebrates. SEM analysis (preparation over the critical point) offers a useful addition to the existing histological preparations. It is suitable for a comparative analysis, in which the arrangement of different cellular eleme...

journal_title：Journal of neuroscience methods

authors： Breidbach O

更新日期：1984-11-01 00:00:00

abstract：:Attempts to correlate the amount of bilateral brain injury with behavioral changes can be complicated if the lesions are bilaterally unsymmetrical in volume. Further complications are introduced if unilateral lesions are ineffective in producing behavioral changes. An index, W%, is proposed which is based on the produ...

journal_title：Journal of neuroscience methods

authors： Hodos W,Bobko P

更新日期：1984-11-01 00:00:00

abstract：:A simple and easily constructed unit was designed using a buffer amplifier, a programmable transconductance amplifier and an active filter. The unit allows extracellular recording and stimulation through the same electrode. Thus in experiments where it is required to stimulate a nerve and record the effects of the sti...

journal_title：Journal of neuroscience methods

authors： Hatzopoulos A,Theophilidis G

更新日期：1984-08-01 00:00:00

abstract：:The increasing use of WGA-HRP as a neuroanatomical tracer has led us to examine two aspects of its uptake and transport. Microelectrophoretic deposits of WGA-HRP were centered in lamina A of the LGd to determine whether or not there is a discrepancy between the size of the deposit site and the effective uptake zone. F...

journal_title：Journal of neuroscience methods

authors： Shook BL,Abramson BP,Chalupa LM

更新日期：1984-06-01 00:00:00

abstract：:A simple and low-cost device is described which facilitates the calibration of intracellular DC recordings in amplitude and time. A current source, optically coupled to a conventional stimulator, injects a pulse of current, across a 1-omega resistor connecting the preparation to ground. Amplitude and duration of the c...

journal_title：Journal of neuroscience methods

authors： Godfraind JM,Veraart C,Nicaise M

更新日期：1984-05-01 00:00:00

abstract：:The relationship between pressure, ejection duration and volume ejected was experimentally determined in vitro for micropipettes with different external tip diameters. The relationship between ejection duration and ejected volume is linear in the steady state (i.e. with ejection durations of 1 s or longer) and at suff...

journal_title：Journal of neuroscience methods

authors： van Dongen PA

更新日期：1984-04-01 00:00:00

abstract：:The properties of 4 types of carbon electrodes designed for use as in vivo sensors of easily oxidized species in the mammalian brain have been evaluated in aqueous solutions at physiological pH. The electrodes are formed from a graphite-epoxy mixture, carbon paste, or carbon fibers, and have the geometries of a disk o...

journal_title：Journal of neuroscience methods

authors： Kovach PM,Ewing AG,Wilson RL,Wightman RM

更新日期：1984-03-01 00:00:00

abstract：:The advantages of utilizing a microprocessor design kit as a central element in custom laboratory instrumentation are demonstrated in the design of a real-time, multiple point, tissue displacement monitor. The tissue sample to be monitored is submerged in a saline bath, through which perpendicular electric fields are ...

journal_title：Journal of neuroscience methods

authors： Looft FJ,Lyons HD

更新日期：1984-02-01 00:00:00

abstract：:A new technique is described for accurate, reliable measurement of eyeball retraction in the rabbit. A narrow film strip, on which a linear light intensity grating has been exposed, is attached to a contact lens which is placed on the animal's cornea. The other end of the intensity grating slides between a photodiode ...

journal_title：Journal of neuroscience methods

authors： Quinn KJ,Kennedy PR,Weiss C,Disterhoft JF

更新日期：1984-01-01 00:00:00

abstract：:The GABA analogue baclofen [3-(p-chlorophenyl)-4-aminobutanoic acid] has stereospecific actions on the peripheral and central nervous systems. This paper describes the resolution of tritium-labelled baclofen by high performance liquid chromatography on a reverse-phase C18 column using a chiral mobile phase. The method...

journal_title：Journal of neuroscience methods

authors： Weatherby RP,Allan RD,Johnston GA

更新日期：1984-01-01 00:00:00

abstract：:A method is described to record the X,Y-coordinates of two bright spots on a TV image. These spots are produced by a light-emitting diodes (LEDs) assembly, which must be affixed on the moving target. The system was developed to record head movements of free-moving animals, chronically implanted to bear a socket LEDs h...

journal_title：Journal of neuroscience methods

authors： Lecas JC,Dutrieux G

更新日期：1983-12-01 00:00:00

abstract：:The assumptions upon which a correct interpretation of the t-test depends are rarely fulfilled by data from the neurosciences. This applies to both independent and correlated samples. The Mann-Whitney U-test is suggested as an alternative for the t-test for independent samples. The way in which significant results fro...

journal_title：Journal of neuroscience methods

authors： Krauth J

更新日期：1983-12-01 00:00:00

abstract：:A new non-metallic push-pull cannula device is described for the localized perfusion of brain sites in the rat or other animal. Comprised entirely of glass and plastic materials, the cannula is intended for usage in an experiment in which the presence in tissue of metal tubing for delivery of perfusate is methodologic...

journal_title：Journal of neuroscience methods

authors： Myers RD,Hepler JR

更新日期：1983-10-01 00:00:00

abstract：:A new simple technique for the accurate modification of microelectrode and micropipette tips by breaking has been developed. The breaking is done by longitudinally inserting a micropipette into a larger previously broken pipette for stability and by applying a force on the micropipette to cause it to break. Tips will ...

journal_title：Journal of neuroscience methods

authors： Briano RA Jr

更新日期：1983-09-01 00:00:00

abstract：:This paper describes a feedback system in which a neutral density wedge automatically controls the light flux applied to a photoreceptor, so that an intracellular recorded response is held constant. We describe here a "differential sampler" that subtracts the photoreceptors dark response from the light response induce...

journal_title：Journal of neuroscience methods

authors： Smakman JG,Pijpker BA

更新日期：1983-08-01 00:00:00

abstract：:The silanization of glass, particularly Pyrex, was studied using reaction conditions that might be applied in the fabrication of ion-sensitive microelectrodes of the liquid-membrane type. The efficacy was tested by measuring the hydrophobicity (contact angle) or electrical resistivity of the treated surface. Aminosila...

journal_title：Journal of neuroscience methods

authors： Munoz JL,Deyhimi F,Coles JA

更新日期：1983-07-01 00:00:00